Tsakiridis, Anestis, Dr

Dr Anestis Tsakiridis

School of Biosciences

Group Leader & Lecturer

a.tsakiridis@sheffield.ac.uk
+44 114 222 2367

Alfred Denny Building

Full contact details

Dr Anestis Tsakiridis
School of Biosciences
Alfred Denny Building
Western Bank
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S10 2TN

Profile

2020-present: Lecturer
2016-2019: Vice Chancellor's Fellow
2009-2015: Post-doctoral researcher, MRC Centre for Regenerative Medicine, University of Edinburgh (Advisor: Prof. Val Wilson)
2006-2009: Post-doctoral researcher, Institute for Stem Cell Research, University of Edinburgh (Advisor: Prof. Josh Brickman)
2002-2006: PhD, University of Edinburgh (Advisors: Prof. Lesley Forrester, Prof. Josh Brickman)
2001-2002: MSc (Res), University of Edinburgh
1998-2001: BSc (Hons), University of Edinburgh

Research interests

My group aims to understand how human embryonic cells adopt different fates as they transit from pluripotency to lineage commitment, and exploit this knowledge for regenerative medicine and disease modelling applications. We focus particularly on defining the determinants of anteroposterior (A-P) axial identity (i.e. the position cells occupy along the head-tail axis) and how this influences cell fate decision-making in various multipotent progenitors of the central and peripheral nervous system. To address our questions we employ predominantly human pluripotent stem cell (hPSCs) differentiation as an in vitro model of early human embryonic development and utilise protocols we have recently established for the production of spinal cord progenitors and neural crest cells (the precursors of various specialised cell types including peripheral neurons) that correspond to distinct axial levels.
Current projects in our lab include:

Understanding how various cellular components of the trunk (e.g. motor neurons, paraxial mesoderm, trunk neural crest) are derived from multipotent axial progenitors
Defining the molecular/signalling basis of A-P axial identity acquisition/maintenance in distinct neural cell types
Deciphering the molecular/signalling logic of cell fate decisions in vagal neural crest derivatives focusing on the enteric nervous system
Determining how abnormal trunk neural crest development is linked to the initiation of neuroblastoma, the most common extra-cranial solid tumour of childhood

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Frith TJR & Tsakiridis A (2019) . Current Protocols in Stem Cell Biology.
Tsakiridis A & Wilson V (2015) . F1000Research, 4.
Tsakiridis A, Huang Y, Blin G, Skylaki S, Wymeersch F, Osorno R, Economou C, Karagianni E, Zhao S, Lowell S & Wilson V (2015) . Development, 142(4), 809-809.
Gouti M, Tsakiridis A, Wymeersch FJ, Huang Y, Kleinjung J, Wilson V & Briscoe J (2014) . PLoS Biology, 12(8).
Tsakiridis A, Huang Y, Blin G, Skylaki S, Wymeersch F, Osorno R, Economou C, Karagianni E, Zhao S, Lowell S & Wilson V (2014) . Development, 141(6), 1209-1221.
Huang Y, Osorno R, Tsakiridis A & Wilson V (2012) . Cell Reports, 2(6), 1571-1578.
Frith TJR, Granata I, Wind M, Stout E, Thompson O, Neumann K, Stavish D, Heath PR, Ortmann D, Hackland JOS , Anastassiadis K et al () . eLife, 7.

All publications

Journal articles

(2024) . Development, 151(3).
Saldana-Guerrero IM, Montano-Gutierrez LF, Hafemeister C, Stavish D, Shaw LE, Fetahu IS, Wenninger-Weinzierl A, Sturtzel C, Souilhol C, Tarelli S , Shoeb MR et al (2023) . Cancer Research, 83(7_Supplement), 3542-3542.
Cooper F & Tsakiridis A (2022) . Developmental Biology.
Cooper F & Tsakiridis A (2022) . Biochem Soc Trans.
Wind M & Tsakiridis A (2021) . Current Protocols, 1(9).
Gogolou A, Frith TJR & Tsakiridis A (2021) . Current Protocols, 1(6).
Wymeersch FJ, Wilson V & Tsakiridis A (2021) . Development, 148(4).
Wind M, Gogolou A, Manipur I, Granata I, Butler L, Andrews PW, Barbaric I, Ning K, Guarracino MR, Placzek M & Tsakiridis A (2021) . Development, 148(6).
Frith TJR, Gogolou A, Hackland JOS, Hewitt ZA, Moore HD, Barbaric I, Thapar N, Burns AJ, Andrews PW, Tsakiridis A & McCann CJ (2020) . Stem Cell Reports.
Ruske LJ, Kursawe J, Tsakiridis A, Wilson V, Fletcher A, Blythe RA & Schumacher LJ (2020) . Physical Biology.
Frith TJR & Tsakiridis A (2019) . Current Protocols in Stem Cell Biology.
Sutherland L, Ruhe M, Gattegno-Ho D, Mann K, Greaves J, Koscielniak M, Meek S, Lu Z, Waterfall M, Taylor R , Tsakiridis A et al (2018) . Journal of Cell Science, 131(18).
Lopez-Yrigoyen M, Fidanza A, Cassetta L, Axton RA, Taylor AH, Meseguer-Ripolles J, Tsakiridis A, Wilson V, Hay DC, Pollard JW & Forrester LM (2018) . Philosophical Transactions of the Royal Society B: Biological Sciences, 373(1750).
Economou C, Tsakiridis A, Wymeersch FJ, Gordon-Keylock S, Dewhurst RE, Fisher D, Medvinsky A, Smith AJH & Wilson V (2015) . BMC Developmental Biology, 15(1).
Tsakiridis A & Wilson V (2015) . F1000Research, 4.
Tsakiridis A, Huang Y, Blin G, Skylaki S, Wymeersch F, Osorno R, Economou C, Karagianni E, Zhao S, Lowell S & Wilson V (2015) . Development, 142(4), 809-809.
Gouti M, Tsakiridis A, Wymeersch FJ, Huang Y, Kleinjung J, Wilson V & Briscoe J (2014) . PLoS Biology, 12(8).
Tsakiridis A, Huang Y, Blin G, Skylaki S, Wymeersch F, Osorno R, Economou C, Karagianni E, Zhao S, Lowell S & Wilson V (2014) . Development, 141(6), 1209-1221.
Huang Y, Osorno R, Tsakiridis A & Wilson V (2012) . Cell Reports, 2(6), 1571-1578.
Tan CL, Tsakiridis A, Noor DAM, Walker DA, Wilson V & Scotting PJ (2012) REASSESSING THE ORIGINS OF INTRACRANIAL GERM CELL TUMOUR FORMATION. PEDIATRIC BLOOD & CANCER, 59(6), 1089-1089.
Osorno R, Tsakiridis A, Wong F, Cambray N, Economou C, Wilkie R, Blin G, Scotting PJ, Chambers I & Wilson V (2012) . Development, 139(13), 2288-2298.
Osorno R, Tsakiridis A, Wong F, Cambray N, Economou C, Wilkie R, Blin G, Scotting PJ, Chambers I & Wilson V (2012) . Journal of Cell Science, 125(12), e1.1-e1.
Brickman JM, Tsakiridis A, To C & Stanford WL (2010) , 271-295.
Tsakiridis A, Tzouanacou E, Rahman A, Colby D, Axton R, Chambers I, Wilson V, Forrester L & Brickman JM (2009) . Nucleic Acids Research, 37(19), e129-e129.
Tsakiridis A, Alexander LM, Gennet N, Sanchez-Martin RM, Livigni A, Li M, Bradley M & Brickman JM (2009) . Biomaterials, 30(29), 5853-5861.
Sanchez-Martin RM, Alexander L, Muzerelle M, Cardenas-Maestre JM, Tsakiridis A, Brickman JM & Bradley M (2009) . ChemBioChem, 10(9), 1453-1456.
Tsakiridis A, Tzouanacou E, Larralde O, Watts TM, Wilson V, Forrester L & Brickman JM (2007) . genesis, 45(6), 353-360.
Jevans B, Cooper F, Fatieieva Y, Gogolou A, Kang Y-N, Restuadi R, Moulding D, Vanden Berghe P, Adameyko I, Thapar N , Andrews PW et al () . Gut, gutjnl-2023.
Saldana-Guerrero IM, Montano-Gutierrez LF, Boswell K, Hafemeister C, Poon E, Shaw LE, Stavish D, Lea RA, Wernig-Zorc S, Bozsaky E , Fetahu IS et al () . Nature Communications, 15(1).
Cooper F, Souilhol C, Haston S, Gray S, Boswell K, Gogolou A, Frith TJR, Stavish D, James BM, Bose D , Dale JK et al () . Development.
Gogolou A, Souilhol C, Granata I, Wymeersch FJ, Manipur I, Wind M, Frith TJR, Guarini M, Bertero A, Bock C , Halbritter F et al () . eLife, 11.
Frith TJR, Granata I, Wind M, Stout E, Thompson O, Neumann K, Stavish D, Heath PR, Ortmann D, Hackland JOS , Anastassiadis K et al () . eLife, 7.
Tsakiridis A & Wilson V () . F1000Research, 4, 100-100.

Conference proceedings papers

Kaviani RS, Shiryaeva M, Souilhol C, Wind M, Tsakiridis A & Luttge R (2023) Enhancing neuronal cell network organization and activity using NOA81 and PDMS nanogrooves. TISSUE ENGINEERING PART A, Vol. 29(13-14)
Frith TJR, McCann C, Gogolou A, Thapar N, Tsakiridis A, Burns A & Andrews PW (2019) Transplantation of hPSC derived enteric neural progenitors as a basis to derive a preclinical cell therapy for Hirschsprung's disease. HUMAN GENE THERAPY, Vol. 30(8) (pp A17-A17)
Wymeersch F, Skylaki S, Huang Y, Tsakiridis A, Economou C, Marek-Johnson C, Tomlinson S & Wilson V (2017) . Mechanisms of Development, Vol. 145 (pp S82-S82)
Tsakiridis A, Tzouanacou E, Rahman A, Axton R, Wilson V, Forrester L & Brickman J (2009) . Mechanisms of Development, Vol. 126 (pp S280-S280)

Preprints

Jevans B, Cooper F, Fatieieva Y, Gogolou A, Kang Y-N, Restuadi R, Berghe PV, Adameyko I, Thapar N, Andrews PW , Coppi PD et al (2023) , Cold Spring Harbor Laboratory.
Cooper F, Souilhol C, Haston S, Gray S, Boswell K, Gogolou A, Frith T, Stavish D, James BM, Bose DA , Dale JK et al (2023) , Cold Spring Harbor Laboratory.
Saldana-Guerrero IM, Montano-Gutierrez LF, Hafemeister C, Stavish D, Shaw LE, Fetahu IS, Wenninger-Weinzierl A, Sturtzel C, Souilhol C, Tarelli S , Shoeb MR et al (2022) , Cold Spring Harbor Laboratory.
Gogolou A, Souilhol C, Granata I, Wymeersch FJ, Manipur I, Wind M, Frith TJR, Guarini M, Bertero A, Bock C , Halbritter F et al (2021) , Cold Spring Harbor Laboratory.
Ruske LJ, Kursawe J, Tsakiridis A, Wilson V, Fletcher AG, Blythe RA & Schumacher LJ (2020) Coupled differentiation and division of embryonic stem cells inferred from clonal snapshots, arXiv.
Butler L, Adamson KI, Johnson SL, Jestice LH, Price CJ, Stavish D, Pooranachandran N, Malicki JJ, Tsakiridis A, Grierson AJ & Barbaric I () , Cold Spring Harbor Laboratory.
Wind M, Gogolou A, Manipur I, Granata I, Butler L, Andrews PW, Barbaric I, Ning K, Guarracino MR, Placzek M & Tsakiridis A () .
Frith TJR, Gogolou A, Hackland JOS, Barbaric I, Thapar N, Burns AJ, Andrews PW, Tsakiridis A & McCann CJ () .

Research group

Katy Boswell, PhD student (jointly with Dan Bose's group, School of Biosciences)
James Birch, PhD student (jointly with Ivana Barbaric group, School of Biosciences)
Fay Cooper, PDRA
Antigoni Gogolou, PhD student
Ingrid Maricel Saldana Guerrero, PDRA (jointly with Helen Bryant's group, Department of Oncology and Metabolism)
Rebecca Lea, PDRA
Sude Uyulgan, PhD student (jointly with Matt Towers' group, School of Biosciences)

We welcome speculative applications. Please email your CV and research interests to: a.tsakiridis@sheffield.ac.uk

Grants

Biotechnology and Biological Sciences Research Council (BBSRC)
The Royal Society
Children's Cancer and Leukaemia Group/Little Princess Trust
MRC Discovery Medicine North Doctoral Training Partnership
EU Horizon2020
Neuroblastoma UK
BBSRC White Rose Mechanistic Biology DTP
Medical Research Council

Teaching activities

Stem cells, ageing and cancer (BMS243/247) (Module joint co-ordinator)
Stem Cell Biology (BMS382/6056)
Literature Review and Research Proposal (BIS405/BMS61005)
Research Project (BMS61006)
Patients as educators/Research project (BIS303)

Professional activities and memberships

Communications Lead for Development, Regeneration and Neuroscience Research Cluster, School of Bioscience, University of ��߲ݴ�ý.
Elected member of the British Society for Developmental Biology committee.
Member of International Society for Stem Cell Research, British Society for Gene and Cell Therapy and Mercia Stem Cell Alliance.
Research Advisory Group, Children's Cancer and Leukaemia Group/Little Princess Trust.
Advisor editor for StemJournal and member of Advisory Board of Cells & Development.

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School of Biosciences

School of Biosciences

Dr Anestis Tsakiridis

Featured publications

Journal articles

All publications

Journal articles

Conference proceedings papers

Preprints

Links

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