Wong, Tuck Seng, Professor

Professor Tuck Seng Wong

BEng(Hons), MSc, PhD (Special distinction), AMIChemE

School of Chemical, Materials and Biological Engineering

Professor of Biomanufacturing

Overseas Admissions Tutor

t.wong@sheffield.ac.uk
+44 114 222 7591

Room C58, Sir Robert Hadfield Building

Full contact details

Professor Tuck Seng Wong
School of Chemical, Materials and Biological Engineering
Room C58
Sir Robert Hadfield Building
Mappin Street
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S1 3JD

Profile

I studied Chemical Engineering at the National University of Singapore (NUS) and graduated with first class honours in 2002. During my undergraduate study, I was awarded Summer Undergraduate Research Fellowship (SURF) by the California Institute of Technology (CalTech) and started working with Prof. Frances H. Arnold on directed protein evolution. My interest in protein science was kindled by this experience. Subsequently, I moved to Germany and completed my MSc and PhD degrees at the Jacobs University Bremen under the supervision of Prof. Dr. Ulrich Schwaneberg. Together with Prof. Schwaneberg, we developed novel tools for directed protein evolution including Sequence Saturation Mutagenesis (SeSaM) and Mutagenesis Assistant Programme (MAP).

Upon completion of my PhD (passed with special distinction) in 2007, I joined the research team of Prof. Sir Alan R. Fersht at Medical Research Council Centre for Protein Engineering (MRC CPE, University of Cambridge). My post-doctoral research encompassed characterizing proteins involved in cancers, ageing, and mutational diseases (particularly tumour suppressor p53 and mitochondrial DNA replisome) using advanced biophysical and structural approaches.

In 2009, I joined the Department of Biological Sciences at the Xi’an Jiaotong-Liverpool University (XJTLU) as an Associate Professor. In 2010, I was promoted to Acting Head of Department. In August 2011, I moved to the University of ��߲ݴ�ý (TUoS) to further advance my academic and research career. I initiated SURF programme in both XJLTU and TUoS to promote undergraduate research.

Research interests

The research in Wong group focuses on applying advanced protein engineering technique, specifically directed evolution, to tailor the properties of enzymes for industrial and pharmaceutical applications as well as to elucidate the design principles used by Nature. There are three key areas that we are currently working on: 1) Development of novel molecular biology tools to advance the field of directed evolution (e.g., method to create high quality mutant library), 2) Application of directed evolution to improve existing properties of industrially relevant enzymes (e.g., cytochrome P450s, carbonyl reductases, aromatic peroxygenases and hydrolases) or to create novel functions, and 3) Development of computational tools to facilitate/expedite experimental design (e.g., method to analyse genetic diversity). One of our current research projects is to develop biological carbon dioxide capture and utilization (CCU) strategies for bulk, fine and specialty chemical syntheses, capitalizing on our interest in directed evolution and synthetic biology.

Complementing protein engineering, we also apply a wide array of biophysical techniques to study various properties of biomolecules (e.g., structure, stability, oligomeric state, protein-protein interaction, and protein-DNA interaction etc). We characterize proteins/complexes involved in cancer, ageing and mutational diseases.

Research topics

Protein engineering (directed evolution)
Biocatalysis and industrial biotechnology
Synthetic biology
Biological carbon dioxide capture and utilization
Biophysics
Cancer and ageing

Research personnel

Pawel Jajesniak (Poland)
Hossam Eldin Omar Ali (United Kingdom)
Amir Zaki Abdullah Zubir (Malaysia)
Abdulrahman H. Alessa (Saudi Arabia)

Publications

Books
Journal articles
Chapters
Other
Preprints

Books

Wong TS & Tee KL (2023) . Springer International Publishing.
Wong TS & Tee KL (2020) . Springer International Publishing.

Journal articles

Sitompul SN, Diaz Garcia LA, Price J, Tee KL & Wong TS (2024) . Biotechnology Journal, 19(7).
Wong TS (2023) . Open Access Government, 39(1), 468-469.
Athamneh K, Alneyadi A, Alsadik A, Wong TS & Ashraf SS (2022) . PLoS ONE, 17(1).
Brojanigo S, Gronchi N, Cazzorla T, Wong TS, Basaglia M, Favaro L & Casella S (2021) . Bioresource Technology, 126383-126383.
Gonzalez-Perez D, Ratcliffe J, Tan SK, Wong MCM, Yee YP, Nyabadza N, Xu J-H, Wong TS & Tee KL (2021) . Scientific Reports, 11.
Unrean P, Napathorn SC, Tee KL, Wong TS & Champreda V (2021) . Biomass Conversion and Biorefinery.
Abdullah Zubir AZ, Whawell SA, Wong TS & Khurram SA (2020) . Oral Diseases.
Johnson AO, Gonzalez-Villanueva M, Wong L, Steinbüchel A, Tee KL, Xu P & Wong TS (2019) . Metabolic Engineering.
Alessa AHA, Tee KL, Gonzalez-Perez D, Omar Ali HEM, Evans CA, Trevaskis A, Xu J-H & Wong TS (2019) . Bioresources and Bioprocessing, 6(1).
Unrean P, Tee KL & Wong TS (2019) . Bioresources and Bioprocessing, 6(1).
González-Villanueva M, Galaiya H, Staniland P, Staniland J, Savill I, Wong TS & Tee KL (2019) . International Journal of Molecular Sciences, 20(22).
Jajesniak P, Tee KL & Wong TS (2019) . International Journal of Molecular Sciences, 20(16).
Tee KL, Xu J-H & Wong TS (2019) . Journal of Biotechnology, 303, 53-64.
Johnson AO, Gonzalez-Villanueva M, Tee KL & Wong TS (2018) . ACS Synthetic Biology, 7(8), 1918-1928.
Tee KL, Grinham J, Othusitse AM, Gonzalez-Villanueva M, Johnson AO & Wong TS (2017) . Biotechnology Journal, 12(11).
Johnson AO, Gonzalez-Villanueva M, Wong L, Steinbüchel A, Tee KL, Xu P & Wong TS (2017) . Metabolic Engineering, 44, 253-264.
Jajesniak P & Wong TS (2016) . Methods in Molecular Biology, 1472, 205-214.
Jajesniak P & Wong TS (2015) . Journal of Biological Engineering, 9, 15-15.
Jajesniak P & Seng Wong T (2015) . AIMS Bioengineering, 2(3), 277-296.
Warburton M, Omar Ali , Liong WC, Othusitse AM, Abdullah Zubir AZ, Maddock S & Wong TS (2015) . AIMS Bioengineering, 2(3), 126-143.
Verma R, Wong TS, Schwaneberg U & Roccatano D (2014) . Methods in Molecular Biology, 1179, 279-290.
Tee KL & Wong TS (2013) . Biotechnology Advances, 31(8), 1707-1721.
Kuper J, Tee KL, Wilmanns M, Roccatano D, Schwaneberg U & Wong TS (2012) . Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 68(9), 1013-1017.
Lim EG, Wang Z, Tillo T, Man KL, Wong TS & Lai KW (2011) . 2011 International SoC Design Conference, ISOCC 2011, 310-312.
Wong TS, Rajagopalan S, Freund SM, Rutherford TJ, Andreeva A, Townsley FM, Petrovich M & Fersht AR (2009) . Nucleic Acids Res, 37(20), 6765-6783.
Di Re M, Sembongi H, He J, Reyes A, Yasukawa T, Martinsson P, Bailey LJ, Goffart S, Boyd-Kirkup JD, Wong TS , Fersht AR et al (2009) . Nucleic Acids Res, 37(17), 5701-5713.
Wong TS, Rajagopalan S, Townsley FM, Freund SM, Petrovich M, Loakes D & Fersht AR (2009) . Nucleic Acids Res, 37(2), 568-581.
Wong TS, Roccatano D, Loakes D, Tee KL, Schenk A, Hauer B & Schwaneberg U (2008) . Biotechnology Journal, 3(1), 74-82.
Kuper J, Wong TS, Roccatano D, Wilmanns M & Schwaneberg U (2007) . J AM CHEM SOC, 129(18), 5786-+.
Wong TS, Roccatano D & Schwaneberg U (2007) . Biotechnology Journal, 2(1), 133-142.
Wong TS, Roccatano D & Schwaneberg U (2007) . Environmental Microbiology, 9(11), 2645-2659.
Wong TS, Roccatano D & Schwaneberg U (2007) . Biocatalysis and Biotransformation, 25(2-4), 229-241.
Roccatano D, Wong TS, Schwaneberg U & Zacharias M (2006) . Biopolymers, 83(5), 467-476.
Wong TS, Schwaneberg U, Sturmer R, Hauer B & Breuer M (2006) A filter paper-based assay for laboratory evolution of hydrolases and dehydrogenases. COMB CHEM HIGH T SCR, 9(4), 289-293.
Wong TS, Zhurina D & Schwaneberg U (2006) . Comb Chem High Throughput Screen, 9(4), 271-288.
Wong TS, Roccatano D, Zacharias M & Schwaneberg U (2006) . Journal of Molecular Biology, 355(4), 858-871.
Schenk A, Wong TS, Roccatano D, Hauer B & Schwaneberg U (2006) Sequence saturation mutagenesis (SeSam) of a gene. BioSpektrum, 12(3), 277-279.
Wong TS, Wu N, Roccatano D, Zacharias M & Schwaneberg U (2005) . Journal of Biomolecular Screening, 10(3), 246-252.
Roccatano D, Wong TS, Schwaneberg U & Zacharias M (2005) . Biopolymers, 78(5), 259-267.
Wong TS, Tee KL, Hauer B & Schwaneberg U (2005) . Analytical Biochemistry, 341(1), 187-189.
Wong TS, Arnold FH & Schwaneberg U (2004) . Biotechnology and Bioengineering, 85(3), 351-358.
Wong TS, Tee KL, Hauer B & Schwaneberg U (2004) . Nucleic acids research, 32(3).
Wong TS & Schwaneberg U (2003) . Current Opinion in Biotechnology, 14(6), 590-596.
Gali VK, Tee KL & Wong TS () . SynBio, 2(2), 142-173.
Keita VM, Lee YQ, Lakshmanan M, Ow DS-W, Staniland P, Staniland J, Savill I, Tee KL, Wong TS & Lee D-Y () . Microbial Cell Factories, 23(1).
Johari YB, Jaffé SRP, Scarrott JM, Johnson AO, Mozzanino T, Pohle TH, Maisuria S, Bhayat‐Cammack A, Lambiase G, Brown AJ , Tee KL et al () . Biotechnology and Bioengineering.
Wong TS () . Journal of Bioprocessing & Biotechniques, 04(03).
Gonzalez-Perez D, Ratcliffe J, Tan SK, Wong MCM, Yee YP, Nyabadza N, Xu J-H, Wong TS & Tee KL () .

Chapters

Jajesniak P, Tee KL & Wong TS (2022) , Methods in Molecular Biology (pp. 123-135). Springer US
Keita VM, Gonzalez-Villanueva M, Wong TS & Tee KL (2020) , Engineering of Microbial Biosynthetic Pathways (pp. 245-302). Springer Singapore
Tee KL & Wong TS (2017) , Directed Enzyme Evolution: Advances and Applications (pp. 201-227). Springer International Publishing
Omar Ali H, Alessa AHA, Al-nuaemi IJ & Wong TS (2017) Protein engineering for lignocellulose degradation In Kumar P, Gurjar BR & Govil JN (Ed.), Environmental Science and Engineering Volume 8: Biodegradation and Bioremediation USA: Studium Press LLC.
Johnson AO, Gonzalez Villanueva M & Wong TS (2017) Engineering Ralstonia eutropha for chemical production In Zhang TC, Gurjar BR & Govil JN (Ed.), Environmental Science and Engineering Volume 10: Industrial Processing & Nanotechnology USA: Studium Press LLC.
Jajesniak P & Wong TS (2017) Tapping into biodiversity: From metagenomics to industrial enzymes In Gurjar BR, Kumar A & Govil JN (Ed.), Environmental Science and Engineering Volume 2: Biodiversity and Conservation USA: Studium Press LLC.
Tee KL & Wong TS (2014) Directed evolution: A powerful algorithm for advancing synthetic biology. In Singh V (Ed.), Biotechnology Volume 4: Applied Synthetic Biology (pp. 465-497). USA: Studium Press LLC.

Other

Wong TS & Tee KL (2024) . Open Access Government, 42(1), 32-33.
Price J, Wong TS & Tee KL (2024) . Open Access Government, 41(1), 378-379.

Preprints

Gali VK, Tee KL & Wong TS (2023) , MDPI AG.
Sitompul SN, Price J, Tee KL & Wong TS (2023) , Cold Spring Harbor Laboratory.
Malika Keita V, Lee YQ, Lakshmanan M, Ow DS-W, Staniland P, Staniland J, Savill I, Tee KL, Wong TS & Lee D-Y (2023) , Cold Spring Harbor Laboratory.
Johari YB, Jaffé SRP, Scarrott JM, Johnson AO, Mozzanino T, Pohle TH, Maisuria S, Bhayat-Cammack A, Brown AJ, Tee KL , Jackson PJ et al () , Cold Spring Harbor Laboratory.
Johari Y, Jaffe S, Scarrott J, Johnson A, Mozzanino TO, Pohle T, Maisuria S, Cammack AB, Brown A, Tee KL , Jackson P et al () , Authorea, Inc..
Tee KL, Jackson PJ, Scarrott JM, Jaffe SRP, Johnson AO, Johari Y, Pohle TH, Mozzanino T, Price J, Grinham J , Brown A et al () .

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School of Chemical, Materials and Biological Engineering

School of Chemical, Materials and Biological Engineering

Professor Tuck Seng Wong

Research topics

Research personnel

Books

Journal articles

Chapters

Other

Preprints

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